AimPlex Multiplexed Immunoassays for Flow

Measure cytokines in single assays or in multiplex panels for human, mouse, rat, non-human primate and canine models.

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How It Works

AimPlex™ multiplex assay technology utilizes multiple bead populations differentiated by size and different levels of fluorescence intensity.  With multiple sizes of beads and multiple levels of fluorescence intensity in each bead size, the AimPlex technology can measure up to 24 analytes simultaneously in a single reaction.  The bead populations in the reaction are determined by a flow cytometer equipped with a 488nm laser.  The maximum emission of the bead classification dye is at 700 nm.

Similar to the principle of a sandwich ELISA, each bead population conjugated with a specific capture antibody traps the protein of interest, such as a cytokine, in the sample. The amount of the analyte captured is detected via a biotinylated antibody against a secondary epitope of the protein, followed by a streptavidin-R-phycoerythrin treatment. The fluorescent intensity of R-phycoerythrin on the beads is quantified on a flow cytometer.  Concentrations of a protein of interest in the samples can be obtained by comparing the fluorescent signals to those of a standard curve generated from a serial dilution of a known concentration of the analyte

The assay procedure consists of a 60 min antigen and capture antibody conjugated bead incubation step, a 30 min biotinylated detection incubation step and a 20 min streptavidin-PE incubation step.